Non Alcoholic Steatohepatitis 1st Edition by Devanand Sarkar ISBN 1071621270 9781071621271

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ISBN 10: 1071621270 
ISBN 13: 9781071621271
Author: Devanand Sarkar

This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. 

Non Alcoholic Steatohepatitis 1st Table of contents:

Histopathological Diagnosis of Nonalcoholic Steatohepatitis (NASH)

Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease

Hydrodynamic Injection for Developing NASH Model

Measurement of Hepatic Lipids

Measurement of Fatty Acid Oxidation in Mammalian Cells

Measurement of In Vivo VLDL and Chylomicron Secretion

Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KC)

Mouse Bone Marrow Cell Isolation and Macrophage Differentiation

Purification and Isolation of Hepatic Stellate Cells

Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes

Culture of Mouse Liver Ductal Organoids

Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived Liver Organoids

Single-Cell RNA Sequencing of NASH Liver Samples

Exosome Isolation and Characterization from Mesenchymal Stem Cells

Extraction and Analysis of Exosomal miRNAs from Mesenchymal Stem Cells

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Tags: Devanand Sarkar, Alcoholic Steatohepatitis