Sale!

DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st Edition by Omar A Oyarzabal, Sophia Kathariou ISBN 1118278674 9781118278673

Original price was: $50.00.Current price is: $35.00.

DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st Edition Omar A. Oyarzabal Digital Instant Download

Author(s): Omar A. Oyarzabal, Sophia Kathariou
ISBN(s): 9781118278673, 1118278674
Edition: 1
File Details: PDF, 3.72 MB
Year: 2014
Language: english
SKU: EB-4746952 Category: Tags: ,

DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st Edition by Omar A Oyarzabal, Sophia Kathariou – Ebook PDF Instant Download/Delivery: 1118278674, 9781118278673
Full download DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st Edition after payment

Product details:

ISBN 10: 1118278674 
ISBN 13: 9781118278673
Author: Omar A Oyarzabal, Sophia Kathariou

Molecular typing of foodborne pathogens has become an indispensable tool in epidemiological studies. Thanks to these techniques, we now have a better understanding of the distribution and appearance of bacterial foodborne diseases and have a deeper knowledge of the type of food products associated with the major foodborne pathogens. Within the molecular techniques, DNA-based techniques have prospered for more than 40 years and have been incorporated in the first surveillance systems to monitor bacterial foodborne pathogens in the United States and other countries. However, DNA techniques vary widely and many microbiology laboratory personnel working with food and/or water face the dilemma of which method to incorporate. DNA Methods in Food Safety: Molecular Typing of Foodborne and Waterborne Bacterial Pathogens succinctly reviews more than 25 years of data on a variety of DNA typing techniques, summarizing the different mathematical models for analysis and interpretation of results, and detailing their efficacy in typing different foodborne and waterborne bacterial pathogens, such as Campylobacter, Clostridium perfringens, Listeria, Salmonella, among others. Section I describes the different DNA techniques used in the typing of bacterial foodborne pathogens, whilst Section II deals with the application of these techniques to type the most important bacterial foodborne pathogens. In Section II the emphasis is placed on the pathogen, and each chapter describes some of the most appropriate techniques for typing each bacterial pathogen. The techniques presented in this book are the most significant in the study of the molecular epidemiology of bacterial foodborne pathogens to date. It therefore provides a unique reference for students and professionals in the field of microbiology, food and water safety and epidemiology and molecular epidemiology.

DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st Table of contents:

Section I: Typing Method, Analysis, and Applications
1 Polymerase Chain Reaction-Based Subtyping Methods
Randomly amplified polymorphic DNA
Amplified fragment length polymorphism (AFLP)
Repetitive-sequence-based PCR
Multiple-locus variable-number tandem repeat analysis
PCR-restriction fragment length polymorphism (PCR-RFLP)
PCR melting profile analysis
References
2 Pulsed-Field Gel Electrophoresis and the Molecular Epidemiology of Foodborne Pathogens
Background
Theory
Parameters critical to PFGE separations
Instrumentation
Epidemiological applications of PFGE
Conclusion
Acknowledgments
References
3 Multilocus Sequence Typing: An Adaptable Tool for Understanding the Global Epidemiology of Bacterial Pathogens
Multilocus sequence typing
MLST databases
Advantages of MLST
Types of MLST schemes
Discriminatory power and epidemiologic concordance
Clonal complexes, epidemic clones, and outbreak clones
Conclusions
Acknowledgments
References
4 High-Throughput Sequencing
Introduction
Existing subtyping methods
WGS: A comprehensive platform for molecular subtyping
MLST versus WGS
SNP analysis versus WGS
Hypervariable markers
Phenotypic markers versus WGS
Technical process of WGS
Computational tools for WGS analysis
WGS in recent foodborne outbreak investigations
Challenges and future prospects of WGS in molecular subtyping
References
5 Analysis of Typing Results
Introduction
Measuring similarity
Creating groupings of related isolates
Comparison of typing methods
References
6 Databases and Internet Applications
Introduction
Existing online networks and resources
Current challenges, possible solutions, and future trends
GeoGenomic identification and an integrated Web-based global infrastructure
References
7 The Transformation of Disease Surveillance, Outbreak Detection, and Regulatory Response by Molecular Epidemiology
Epidemiology and surveillance
Outbreaks
PulseNet
From steps to principles
The end of the culture era?
Summary
Acknowledgments
References
Section II: Pathogens
8 The Genus Bacillus
Bacillus: A highly heterogeneous genus challenging food quality and safety
Bacillus toxins are gaining increasing prominence as causative agents of foodborne diseases
Polymerase chain reaction methods
Multilocus sequence typing (MLST) and amplified fragment length polymorphism (AFLP): The “golden standards” for population analysis of the B. cereus group
Pulsed-field gel electrophoresis
Microarrays and high-throughput sequencing: From genotyping to genomotyping
Conclusions and future direction
References
9 Molecular Typing of Campylobacter jejuni
Introduction
Brief history of typing methods to study C. jejuni
Most common methods to type C. jejuni
Less commonly used typing methods
Comparative genomic fingerprinting
Combination of techniques
References
10 DNA Typing Methods for Members of the Cronobacter Genus
Introduction
Cronobacter pathogenicity and virulence
Taxonomy and genomic diversity of the Cronobacter genus
Cronobacter and the food industry
Biotyping of Cronobacter strains
DNA-based typing of Cronobacter strains
Multilocus sequence typing of Cronobacter spp.
Case studies of using DNA sequence–based typing of Cronobacter spp.
Current issues in the application of DNA typing methods for Cronobacter spp.
Conclusions
Acknowledgments
References
11 Molecular Subtyping Approaches for Pathogenic Clostridium spp. Isolated from Foods
Introduction
Concluding remarks
Disclaimer
References
12 Molecular Characterization of Shiga Toxin-Producing Escherichia coli
Introduction
DNA fingerprinting
Sequence-based genotyping
Virotyping
Conclusions
References
13 Molecular Subtyping Methods for Listeria monocytogenes: Tools for Tracking and Control
Introduction
Fragment-based methods
Hybridization-based methods
DNA sequence-based subtyping methods
Acknowledgments
References
14 Salmonella
Introduction
Restriction analysis-based genotyping
PCR-based typing methods
DNA sequencing-based typing methods
Comparison of molecular subtyping methods for Salmonella
Conclusions
Disclaimer
References
15 Vibrio cholerae
Introduction
CTX Phage
CTXcla (classical type CTX) and CTX-1 (El Tor type CTX or CTXEl Tor)
CTX-2
CTX-3
ctxB Typing
TLC element
Genotyping of V. cholerae
MLVA analysis of V. cholerae O1 strains

People also search for DNA Methods in Food Safety Molecular Typing of Foodborne and Waterborne Bacterial Pathogens 1st:

dna methods in food safety
    
dna food examples
    
dna protecting foods
    
dna food analysis
    
dna in food

Tags:

Omar A Oyarzabal,Sophia Kathariou,DNA Methods,Food Safety,Molecular Typing,Foodborne,Waterborne Bacterial