Single Molecule Enzymology Methods and Protocols 1st Edition by Alex E Knight, Gregory I Mashanov, Christopher Batters ISBN 1617792616 9781617792618

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ISBN 10: 1617792616 
ISBN 13: 9781617792618
Author: Alex E Knight, Gregory I Mashanov, Christopher Batters

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy.  In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Single Molecule Enzymology Methods and Protocols 1st Table of contents:

1. Single Enzyme Studies: A Historical Perspective
   Alex E. Knight

2. Functional Surface Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins
   Volker Bormuth, Felix Zörgibel, Erik Schäffer, Jonathon Howard

3. Studying Kinesin’s Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer
   Zdenek Lansky, Erwin J. G. Peterman

4. Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors
   Melinda Tonks Hoffman, Janet Sheung, Paul R. Selvin

5. Snapshots of Kinesin Motors on Microtubule Tracks
   Franck J. Fourniol, Carolyn A. Moores

6. Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy
   Frédéric Eghiaian, Iwan A. T. Schaap

7. Using Optical Tweezers to Study the Fine Details of Myosin ATPase Mechanochemical Cycle
   Christopher Batters, Claudia Veigel

8. Quantum Dot Labeling Strategies to Characterize Single-Molecular Motors
   Shane R. Nelson, M. Yusuf Ali, David M. Warshaw

9. Imaging Individual Myosin Molecules Within Living Cells
   Tatiana A. Nenasheva, Gregory I. Mashanov, Michelle Peckham, Justin E. Molloy

10. Single-Molecule Measurements Using Microneedles
    Toshio Yanagida, Yoshiharu Ishii, Akihiko Ishijima

11. Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology
    Christopher P. Toseland, Martin R. Webb

12. Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
    Christoph G. Baumann, Stephen J. Cross

13. A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases
    Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, Justin E. Molloy

14. Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes
    Ryo Iizuka, Takashi Funatsu, Sotaro Uemura

15. Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers
    Yeonee Seol, Keir C. Neuman

16. Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single-Molecule Imaging
    Ja Yil Lee, Eric C. Greene

17. Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor
    Takayuki Nishizaka, Yuh Hasimoto, Tomoko Masaike

18. Microsecond Resolution of Single-Molecule Rotation Catalyzed by Molecular Motors
    Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, Wayne D. Frasch

19. Erratum to: A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases
    Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, Justin E. Molloy

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Tags: Alex E Knight, Gregory I Mashanov, Christopher Batters, Enzymology