Protein Targeting Protocols Methods in Molecular Biology Vol 390 2nd Edition by Mark Van Der Giezen ISBN ‎1617377279 978-1617377273

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Product details: 

ISBN 10: ‎ 1617377279

ISBN 13:  978-1617377273

Author:  Mark Van Der Giezen

In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.

Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.

Table of contents: 

Part I: Bacterial Systems

1. Front Matter

2. Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching
   Conrad W. Mullineaux

3. Membrane Protein Insertion and Secretion in Bacteria
   Jeanine de Keyzer, Martin van der Laan, Arnold J.M. Driessen

4. Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria
   Yihfen T. Yen, Christos Stathopoulos

5. Periplasmic Targeting of Recombinant Proteins in Escherichia coli
   Filipe J. Mergulhão, Gabriel A. Monteiro

6. In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export
   Michael Moser, Sascha Panahandeh, Eva Holzapfel, Matthias Müller

Part II: Eukaryotic Systems

1. Front Matter

2. Purification of a Functional Mammalian SRP54 Core Protein by Heterologous Expression in Escherichia coli
   Markus Pool, Bernhard Dobberstein

3. Targeting of Proteins to the Endoplasmic Reticulum
   Andreas Nyathi, Stephen High

4. Isolation of Functional Translocation Complexes of Yeast Sec61 Complex
   Karin Römisch, Sandra Schwappach

5. Isolation and Characterization of Plant Sec61 Complex
   Viktor I. Korolev, Oliver Trösch, Enrico Schleiff

6. Protein Import into the Endoplasmic Reticulum in a Plant Cell-Free System
   Birgit C. Lingner, Enrico Schleiff

7. Protein Import into Chloroplasts
   Enrico Schleiff, Dirk Schnell

8. Protein Targeting to Mitochondria
   Nils Wiedemann, Nikolaus Pfanner

9. Protein Translocation into Peroxisomes
   Ralf Erdmann, Gabriele Dodt

10. Protein Targeting to the Nucleus
    Dirk Görlich, Ulrike Kutay

11. Nuclear Export of Proteins and RNAs
    Frauke Melchior, Irmgard Hauber

12. Protein Targeting to the Plasma Membrane and the Secretory Pathway
    Hans-Peter Hauri, Thomas Wieland

13. Targeting and Recycling of Endosomal Proteins
    Joachim Füllekrug, Hans-Peter Hauri

Part III: Methods for Studying Protein Targeting and Translocation

1. Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening
   Takeaki Ozawa, Yoshio Umezawa

2. Trafficking Through the Early Secretory Pathway of Mammalian Cells
   Theresa H. Ward

3. Studying Protein Export From the Endoplasmic Reticulum in Plants
   Sally L. Hanton, Loren A. Matheson, Federica Brandizzi

4. Imaging the Golgi Apparatus in Living Mitotic Cells
   Nihal Altan-Bonnet

5. Evaluating Yeast Biosynthetic Vacuolar Transport
   Brian A. Davies, Darren S. Carney, Bruce F. Horazdovsky

6. Targeting to Lysosomes in Mammalian Cells
   Ann H. Erickson, Jeffrey P. Bocock

7. Monitoring Autophagy in Yeast
   Daniel J. Klionsky

8. Protein Targeting to Yeast Peroxisomes
   Ida van der Klei, Marten Veenhuis

9. Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins
   Jeanne Shepshelovich, Koret Hirschberg

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Tags: Mark Van Der Giezen, Protein Targeting Protocols, Methods in Molecular