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Drosophila Cytogenetics Protocols 1st Edition by Daryl S Henderson ISBN 1588290506 9781588290502

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Drosophila Cytogenetics Protocols 1st Edition Daryl S. Henderson Digital Instant Download

Author(s): Daryl S. Henderson
ISBN(s): 9781588290502, 1588290506
Edition: 1
File Details: PDF, 6.75 MB
Year: 2003
Language: english
SKU: EB-923300 Category: Tag:

Drosophila Cytogenetics Protocols 1st Edition by Daryl S Henderson – Ebook PDF Instant Download/Delivery: 1588290506, 9781588290502
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ISBN 10: 1588290506 
ISBN 13: 9781588290502
Author: Daryl S Henderson

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Drosophila Cytogenetics Protocols 1st Table of contents:

Chapter 1: Introduction to Drosophila Cytogenetics

1.1 Historical Background
1.2 Importance of Drosophila in Genetics Research
1.3 Overview of Drosophila Genome and Chromosomes
1.4 Basic Cytogenetic Techniques

Chapter 2: Drosophila Culture and Maintenance

2.1 Fly Strains and Genetic Stocks
2.2 Media Preparation and Handling
2.3 Rearing Conditions and Temperature Effects
2.4 Handling and Transfer of Flies
2.5 Common Issues in Fly Cultures

Chapter 3: Drosophila Chromosome Structure and Analysis

3.1 Polytene Chromosomes: Structure and Features
3.2 Mitotic Chromosomes: Preparation and Visualization
3.3 Chromosomal Banding Patterns and Mapping
3.4 Chromosomal Aberrations and Mutations

Chapter 4: Cytogenetic Techniques and Protocols

4.1 Slide Preparation for Polytene Chromosomes
4.2 Squash and Staining Techniques
4.3 Fluorescence In Situ Hybridization (FISH)
4.4 Chromosome Imaging and Documentation

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Tags: Daryl S Henderson, Drosophila Cytogenetics